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Date May 2019 Marks available 2 Reference code 19M.3.HL.TZ2.11
Level Higher level Paper Paper 3 Time zone Time zone 2
Command term Determine Question number 11 Adapted from N/A

Question

A segment of DNA is shown. Determine a possible open reading frame (ORF) segment in the DNA segment by completing the table.

[2]
a.

In gene research, outline the use of open reading frames.

[1]
b.i.

In gene research, outline the use of gene knockout.

[1]
b.ii.

In gene research, outline the use of BLASTn.

[1]
b.iii.

Markscheme

a. correct starting point ✔
eg

OR

b. correct RNA nucleotides ✔

a.

identify a sequence/gene «that has the potential to be transcribed» ✔

b.i.

silence gene to observe the effect when the gene is not expressed
OR
change in phenotype to deduce function of gene ✔

b.ii.

to compare nucleotide/DNA sequence with other «nucleotide» sequences ✔

b.iii.

Examiners report

Most candidates had difficulty completing the diagram and/or used DNA nucleotides instead of RNA nucleotides.

a.

Knowledge of different tools in gene research seems to be fine, but gene knockout was less understood.

b.i.

Knowledge of different tools in gene research seems to be fine, but gene knockout was less understood.

b.ii.
[N/A]
b.iii.

Syllabus sections

Option B: Biotechnology and bioinformatics » Option B: Biotechnology and bioinformatics (Core topics) » B.2 Biotechnology in agriculture
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Option B: Biotechnology and bioinformatics » Option B: Biotechnology and bioinformatics (Core topics)
Option B: Biotechnology and bioinformatics

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